General Cell Based Assays
Cell Biolabs Inc OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence), 96 assays
Cell Biolabs’ OxiSelect™ Intracellular ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, or other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescin diacetate (DCFH-DA). In brief, DCFH-DA is diffused into cells and is deacetylated by cellular esterases to non-fluorescent 2’, 7’-Dichlorodihydrofluorescin (DCFH), which is rapidly oxidized to highly fluorescent 2’, 7’-Dichlorodihydrofluorescein (DCF) by ROS. The fluorescence intensity is proportional to the ROS levels within the cell cytosol. The effect of antioxidant or free radical compounds on DCF-DA can be measured against the fluorescence of the provided DCF standard. The kit has a DCF detection sensitivity limit of 10 pM. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Cayman Chemical MitoROS™ 580, 500 test
A fluorescent probe for mitochondrial superoxide production in live cells; cell-permeable and selectively targets mitochondria; upon oxidation by superoxide, MitoROS™ 580 fluoresces, and this fluorescence can be measured using fluorescence microscopy or flow cytometry; ex/em = 510/580 nm; has been used to determine effects of curcumin treatment on superoxide production in Jurkat cells
Eurofins Discoverx PathHunter Human GPR25 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR25 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR25 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human EpoR-JAK2 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human EpoR-JAK2 Stable Cell Line Functional Kinase Assay (U2OS) measures EpoR cytokine receptor activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Human IL-1 ASY
The Meso Scale Discovery U-PLEX Human IL-1alpha Assay Kit is a multiplex immunoassay that measures IL-1alpha in human serum plasma and cell culture supernatant samples The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and diluents The assay has a typical sensitivity of 0 04 pg/mL and an upper limit of quantification of 2500 pg/mL
Cayman Chemical RXR RetInoId X receptr100uL
A KCC2 cotransporter activator (EC50 616 nM for chloride transport in NG108-cl cells) selective for KCC2 over GABAA receptors at 50 UM increases the rate of chloride accumulation in spinal slices isolated from a rat model of PNI and BDNF control slices increases mechanical withdrawal thresholds in a rat model of PNI and reverses morphine-induced hyperalgesia in rats at 100 mg/kg
Eurofins Discoverx PathHunter Human LTB4R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human LTB4R β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure LTB4R (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress GALR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0759E2CP2L)
The PathHunter GALR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR2 and EA-tagged β-Arrestin-2. Activation of GALR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Apicidin, HDAC inhibitor, 1MG
MW 623.8 Da, Purity >95%. Potent histone deacetylase inhibitor (IC₅₀ = 0.7 nM). Inhibits cancer cell proliferation via induction of p21WAF1/Cip1 and gelsolin. Active in vitro and in vivo. Cell-permeable.
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery Canine ProInflammatory Panel 3 Ultra-Sensitive Kit
This item has a minimum order quantity of 5 packs per supplier requirements. Cytokines & Chemokines , Immunology/Inflammationm, Canine, 96 wells